ABSTRACT
Objective To detect the significance of the SHP-1,p15 and SOCS-1 genes methylation status in the genesis and development of diffuse large B-cell lymphoma (DLBCL).Methods The methylation state of SHP-1,p15 and SOCS-1 genes CpG islands were measured by methylation-specific polymerase chain (MSP) reaction.Results The positive rates of aberrant methylation for SHP-1,p15 and SOCS-1 genes in 50 specimens of DLBCL were 96 ％ (48/50),52 ％ (26/50) and 56 ％ (28/50) respectively.In control group,however,15 specimens of benign lymph node proliferation showed no methylation in CpG island of SHP-1,p15 and SOCS-1 genes promoter.Conclusion Aberrant methylations of the SHP-1,p15 and SOCS-1 genes exist in the patients with DLBCL.The methylations of SHP-1,p15 and SOCS-1 genes may be associated with the occurrence and development of DLBCL.This study provides a theoretical basis of treatment methylation for DLBCL.
ABSTRACT
Objective To investigate the distribution features and antibiotic drug resistance of infection pathogens isolated from elderly patients with malignancies complicated with infection following treatment in an open hematology ward.Methods From January 2010 to June 2012,specimens from hospitalized elderly patients with concurrent infection were cultured to isolate infection pathogens through routine methods.Antimicrobial susceptibility tests were performed by the microdilution method.Results A total of 302 strains of infection pathogens were isolated from all detected samples,among which isolated strains of Gram-positive bacteria,Gram-negative bacteria and fungi were 91,194 and 17 strains,respectively.Isolated fungi were mostly susceptible to antifungal drugs.Gram-negative bacteria were highly susceptible to carbapenem,while Gram-positive bacteria were most sensitive to vancomycin and teicoplanin.Conclnsion The nosocomial infection pathogens of elderly patients with malignancies in hematology ward are most likely Gram-negative bacteria.Before specimens culture results and susceptibility are known,all these patients should be empirically treated with broad spectrum antibiotics,which have the most activity against Gram-negative bacteria as well as Gram-positive bacteria coverage.